Topic of Research Seminar: Modern mass spectrometric approaches in understanding the complexity of disease: beta-thalassemia as an example
Abstract: Textile market is an important economy builder globally. The unpresidential increase of textile material fouling causes the textile market leads to major concern regarding the development of antifouling finished textile. Textile wears next to the skin, thus upon fouling contributes a major role in the spread of infectious diseases. Especially the hospital acquired infections due to contamination of medical textile surfaces leads major health challenges. Nanotechnology has the potential for developing nanoencapsulated antifouling functional finishing for textile render antibacterial/ antimicrobial. In consideration of this, nanoprecipitation technique was employed to develop antifouling polymeric formulation (APF) for the slow release of antifouling activity. In the first phase cefotaxime as antifouling functional moiety was selected to encapsulated inside the biodegradable polymeric shell. The optimization of blank and drug loaded polymeric nano formulation was done by varying the concentration of aqueous and organic phases. Initially the aqueous phase was optimized by dissolving the varying concentration of polyvinyl alcohol (PVA) as nonionic surfactant in 10 ml deionized water and organic phase was optimized by varying concentration of polycaprolactone PCL in 2 ml dichloromethane DCM. After optimizing aqueous /organic phases, the other process parameters, (stirring time, stirring time, temperature, injection rate) was optimize. The choice of surfactant and the aqueous /organic ratios along with various process parameters were the canons for stability and the size polymeric nanoparticles. The optimized blank and drug loaded formulation was characterized for average size charge and zeta potential through dynamic light scattering DLS. The size and charge of the blank and drug loaded polymeric nanoparticles was found to be 200 nm and – 6.8 mV while ,216 nm and -11.2 mV respectively. Surface and structural morphology was confirmed through scanning electron microscopy SEM and Fourier transform infrared spectroscopy FTIR. In- itro antibacterial acyivity was analyzed against two clinical strains Escherichia coli (ATCC 8739) Staphylococcus aureus (ATCC 6538), through well diffusion assay with 99.9 % reduction in bacterial colonies. In the second phase the nonencapsulated antifouling polymeric formulation (AFP) was coated on cotton textile fabric through layer-by-layer self-assembled multilayers coating. Therefore, antibacterial finishing was employed on cotton textile substrate by alternate dipping in oppositely charges polyelectrolytes solution. The textile fabric was initially dipped in poly (diallyl dimethyl ammonium chloride) (PDAC) solution followed by washing twice in two separate beakers to furnish a single layer of positive charge and then dipping in antifouling polymeric formulation (APF) followed by subsequent washing to accomplish a stable 1 bilayer of opposite charges. The deposition of 1 to 20 bilayers were deposited by repeated the cyclical procedure that enhance the roughness. The coated and uncoated textile samples were characterized for surface morphology, roughness, and thickness through scanning electron microscopy SEM, Optical Profilometery OP and Atomic force microscopy AFM. The surface hydrophobicity was analyzed through drop shape analyzed by measuring contact angle ?, which varies significantly with subsequent adoption of 1 to 20 bilayers. The in-vitro antibacterial assay was qualitatively analyzed against two bacterial strains gram-positive S. aureus and gram-negative E. coli through agar disk diffusion. The significant zone of inhibition was observed against each strain around the coated textile sample as a function of concentration and deposition number of bilayers. The resultant antifouling finished textile has potential to use in biomedical and industrial applications. Moreover, the antifouling polymeric formulation (APF) could potential be highly useful for various type of textile substate beside cotton.
Subject Field of Topic: Bio-analytical Chemistry
Name of Speaker: Prof. Dr. Syed Ghulam Musharraf
Professorial Rank of Speaker: Professor at University of Karachi
University Email of Speaker: [email protected]
Affiliation of Speaker: H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi
Date and Venue: February 16, 2023 at 1400 hrs, at School of Mechanical and Manufacturing Engineering (SMME) Seminar Hall, NUST Islamabad Campus